A cell culture system for studying kidney stone disease

Authors

  • M. Heimmel Department of Genetics, Rutgers University, Piscataway, New Jersey, 08854
  • D. Glass Department of Genetics, Rutgers University, Piscataway, New Jersey, 08854
  • L. Wang Department of Genetics, Rutgers University, Piscataway, New Jersey, 08854
  • A. Sahota Department of Genetics, Rutgers University, Piscataway, New Jersey, 08854
  • J. A. Tischfield Department of Genetics, Rutgers University, Piscataway, New Jersey, 08854

Keywords:

kidney stone disease, cell culture system, cDNA arrays, gene expression changes

Abstract

Background Adenine phosphoribosyltransferase (APRT) deficiency is associated with 2,8-dihydroxyadenine (DHA) kidney stone disease in humans and in a mouse model for this disease. The renal deposition of DHA is associated with the expression of genes involved in tissue injury. To determine the molecular basis for tissue injury, we investigated gene expression alterations in cultured normal human kidney cortical epithelial (NHK-C) cells exposed to DHA crystals.


Methods First strand cDNAs were synthesized from mRNAs isolated from treated and untreated cells. These cDNAs were hybridized to membrane-bound human cDNA arrays, and the relative changes in gene expression in treated versus untreated cells were quantified.


Results The expression of four genes, -catenin, integrin 3, and integrin 6, and platelet-derived growth factor B (PDGF-B) was elevated following exposure of NHK-C cells to DHA.


Conclusions These findings suggest that DHA crystals stimulate the expression of specific genes in cultured renal epithelial cells. DHA crystals may affect cell-cell or cell-matrix interactions or alter the cell structure, and these changes may ultimately contribute to renal injury.

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Author Biography

M. Heimmel, Department of Genetics, Rutgers University, Piscataway, New Jersey, 08854

Rutgers Undergraduate Research Fellow

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Published

2001-09-30

How to Cite

Heimmel, M. ., Glass, D. ., Wang, L. ., Sahota, A. ., & Tischfield, J. A. . (2001). A cell culture system for studying kidney stone disease. The Rutger Scholar, 3. Retrieved from https://rutgersscholar.libraries.rutgers.edu/index.php/scholar/article/view/39

Issue

Vol. 3 (2001)

Section

Articles